Analysis of Met aminoacyl synthetase.

Analysis performed on 2024-07-10 11:39:55

Number of Proteins Analyzed

Initial Filtered Out Final Blast
Bacteria 48220 4310 43910 26233
Archaea 3853 468 3385 1384
Vertebrata 1491 656 835 429
Mammals 471 54 417 133

Number of Variants Analyzed:

Protein Length Pathogenic Variants Benign/Unknown Variants
900 54 772

Domains

Domains
Current Study 1, 256
Guo et al (2010) 8, 218 | 844, 897

Results

Mutations in Ancient vs Modern Domains

Current Study

Ancient Domains Modern Domains All Ratio
Number of Residues 645 255 900 2.529412
Number of Variants 568 258 826 2.201550
Number of Pathogenic Variants 46 8 54 5.750000
Number of Benign/Unknown Variants 522 250 772 2.088000
Fisher exact test 0.006028

The Fisher exact test was calculated using a contingency table with the number of pathogenic and benign/unknown variants in ancient and modern domains. The bottom left values.

Guo et al (2010)

Ancient Domains Modern Domains All Ratio
Number of Residues 635 265 900 2.396226
Number of Variants 552 274 826 2.014599
Number of Pathogenic Variants 43 11 54 3.909091
Number of Benign/Unknown Variants 509 263 772 1.935361
Fisher exact test 0.050832

The Fisher exact test was calculated using a contingency table with the number of pathogenic and benign/unknown variants in ancient and modern domains. The bottom left values.

Coverage of the alignment and domain locations

Agreement between the current study and Guo et al (2010)

Conservation of the amino acids in modern and ancient domains

# of sites Ancient # sites Modern Mean Ancient (SD) Mean Modern (SD) Median Ancient Median Modern Mann-Whitney U p-value
Current Study 645 255 0.74 (0.01) 0.69 (0.01) 7.352e-01 6.919e-01 94172.50 3.42e-04
Guo et al (2010) 635 265 0.73 (0.01) 0.70 (0.01) 7.337e-01 6.959e-01 94154.50 2.42e-03

The Mann-Whitney U test was calculated using the conservation scores of the amino acids in ancient and modern domains. The p-value is for the null hypothesis that the conservation scores in ancient domains are greater than in modern domains.

Conservation of the amino acids in modern and ancient domains